IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
artículos
Título:
Reduced embryo sensitivity to abscisic acid in a sprouting susceptible
Autor/es:
NICOLÁS GUALANO; FERNANDO CARRARI; MARÍA VERÓNICA RODRÍGUEZ; LAURA PÉREZ-FLOREZ; RODOLFO SÁNCHEZ; NORBERTO D. IUSEM; ROBERTO BENECH-ARNOLD
Revista:
SEED SCIENCE RESEARCH
Editorial:
Cambridge University Press
Referencias:
Lugar: Cambridge (UK); Año: 2007 vol. 17 p. 81 - 90
ISSN:
0960-2585
Resumen:
In the work reported in this paper, we attempted to
elucidate the nature of the different abscisic acid
(ABA) sensitivities presented by developing embryos
from sorghum [Sorghum bicolor (L.) Moench] lines
with contrasting pre-harvest sprouting (PHS) behaviour
(Redland B2, susceptible; IS 9530, resistant). We
explored two different hypotheses for a possible
mechanism: (1) a different functionality of the ABA
signalling pathway, and (2) a different rate of ABA
degradation/conjugation in the apoplast of embryos
from these genotypes. To assess the first possibility,
we used an ABA-responsive gene (Rab17) as a
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
with contrasting pre-harvest sprouting (PHS) behaviour
(Redland B2, susceptible; IS 9530, resistant). We
explored two different hypotheses for a possible
mechanism: (1) a different functionality of the ABA
signalling pathway, and (2) a different rate of ABA
degradation/conjugation in the apoplast of embryos
from these genotypes. To assess the first possibility,
we used an ABA-responsive gene (Rab17) as a
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
with contrasting pre-harvest sprouting (PHS) behaviour
(Redland B2, susceptible; IS 9530, resistant). We
explored two different hypotheses for a possible
mechanism: (1) a different functionality of the ABA
signalling pathway, and (2) a different rate of ABA
degradation/conjugation in the apoplast of embryos
from these genotypes. To assess the first possibility,
we used an ABA-responsive gene (Rab17) as a
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
Sorghum bicolor (L.) Moench] lines
with contrasting pre-harvest sprouting (PHS) behaviour
(Redland B2, susceptible; IS 9530, resistant). We
explored two different hypotheses for a possible
mechanism: (1) a different functionality of the ABA
signalling pathway, and (2) a different rate of ABA
degradation/conjugation in the apoplast of embryos
from these genotypes. To assess the first possibility,
we used an ABA-responsive gene (Rab17) as a
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17
Rab17) as a
reporter of changes in endogenous ABA content,
which were artificially induced in embryos from both
genotypes by means of fluridone application immediately
after anthesis, to reduce ABA content, and
embryo incubation in the presence of ABA. A defect in
ABA signalling should be seen as a level of Rab17Rab17
expression that is independent of endogenous ABA
content. For testing the second possibility at two
stages of development, embryos from both lines were
isolated and incubated in water for different periods.
ABA concentrations in embryos and the incubation
media were quantified through radioimmunoassay. In
contrast to our findings for the resistant IS 9530 line,
Rab17 expression did not respond to changes in ABA
levels in sensitive Redland B2 embryos. The ABA
degradation/conjugation rates in embryos and incubation
media did not show clear differences between
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
levels in sensitive Redland B2 embryos. The ABA
degradation/conjugation rates in embryos and incubation
media did not show clear differences between
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
levels in sensitive Redland B2 embryos. The ABA
degradation/conjugation rates in embryos and incubation
media did not show clear differences between
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
expression did not respond to changes in ABA
levels in sensitive Redland B2 embryos. The ABA
degradation/conjugation rates in embryos and incubation
media did not show clear differences between
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.
sorghum lines for any of the developmental stages
analysed. These results suggest that a disruption in
the ABA signal transduction pathway in Redland B2
underlies the low ABA sensitivity shown by embryos
from this line.