Interaction of a Peripheral Acidic Protein with Anionic Lipid Membranes Insights from Molecular Simulations

VANESA V. GALASSIA, MARCOS VILLARREAL, MARIANA BOLLO, GRACIELA BERBERIÁN, LUIS BEAUGÉ, GUILLERMO G. MONTICH

Congreso; XXXIX Reunión Anual de la Sociedad Argentina de Biofísica; 2010

ReP1 belongs to the FABP family, sharing the common terciarymotif of -barrel. This soluble protein up-regulates the Na+/Ca2+exchanger of squid. It has been found to interact with anionic lipidssuch as phosphatidilinositols and phosphatidic acids [1].Its isoelectric point is 5.85, which leads to a net charge of -1.This, together with its ability to interact with anionic lipids, makes ita potential model to test the hypothesis that the binding and theorientation of peripheral proteins to lipid membranes is driven bythe interaction of its macrodipole with the electric field of theinterface [2]