Interaction of nanoparticles with organized systems that are of interest in nanobiosciences

MARTÍN E. VILLANUEVA; RAQUEL V. VICO

Golden, Colorado

Workshop; ACS Summer School on Green Chemistry and Sustainable Energy; 2018

American Chemical Society (ACS)

Nanoparticles (NPs) represent one of the most relevant platforms used for biomedical applications. The use of this nanostructures offers many advantages due to their unique size, physical properties and also, the versatility to be functionalized with molecules of different nature which act as stabilizers and active surface agents at the same time. In order to achieve the insertion of these systems in living organisms for medical purposes, it is necessary to know the bio-physicochemical bases of the interaction between NPs and biological structures. Biomembrane models such as Langmuir Monolayers and Lipid Vesicles comprise traditional techniques in colloid and interface science to aboard this studies. In our work, we have synthesized and fully characterized silver (AgNPs) and magnetic (MNPs) nanoparticles, coated by oleic acid (OA) to explore the interaction between NPs and phospholipids by means of biomembrane models in order to know the factors involved in such interactions and make possible the future projection of applications in biomedicine.Our results suggest that oleic acid coated silver nanoparticles (AgNPs@OA) have a strong insertion on membranes (according to thermodynamical and topographical data obtained in Langmuir films experiments) and once in there, they can release the required doses of silver ions (Ag+) to exert an antimicrobial activity (biocide effect). On the other side, oleic acid coated magnetic nanoparticles (MNPs@OA) were able to induce bilayer merging processes (fusion) on lipid vesicles that can be regulated by the application of variable intensity magnetic fields near the system.