INFIQC   05475
INSTITUTO DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Quaternized chitosan as support for the assembly of gold nanoparticles and evaluation of its biocatalytic activity
Autor/es:
MARIA V. BRACAMONTE, SOLEDAD BOLLO, PIERRE LABBÉ, GUSTAVO A. RIVAS, NANCY F. FERREYRA
Revista:
ELECTROCHIMICA ACTA
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2010 vol. 56 p. 1316 - 1322
ISSN:
0013-4686
Resumen:
Wereport for the first time the use of quaternized chitosan (QCHI) for the immobilization of gold nanoparticles
(NP) and glucose oxidase (GOD), the characterization of the resulting platform and its biocatalytic
activity using glucose as substrate. The chemical substitution of chitosan has allowed us to work at physiologic
pH to build up self-assembled layers of QCHI-NP as platform for the enzyme immobilization. The
adsorption of GOD was analyzed by surface plasmon resonance (SPR) to compare the surface coverage
of GOD in absence and presence of the QCHI-NP platform. The results obtained with cyclic voltammetry
and scanning electrochemical microscopy (SECM) revealed that the adsorption of NP improves the
conductivity of the structure and its electrochemical reactivity, facilitating the oxidation of the hydrogen
peroxide produced by GOD. The electrodes modified with NP present higher amperometric response
demonstrating the efficient transduction of the enzymatic activity in this structure