Self-assembled multilayers of polyethylenimine and DNA: spectrophometric and electrochemical characterization and application for the determination of AO interaction

N. F. FERREYRA, G. A. RIVAS

ELECTROANALYSIS

Wiley-VCH

Año: 2008

1040-0397

This work deals with the study of the interaction between acridine orange (AO) and calf-thymus double stranded DNA (dsDNA) present in supramolecular architectures built on gold electrodes modified with mercapto-1-propanesulfonic acid (MPS) by self-assembling of polyethylenimine and dsDNA. The optimal conditions for building the supramolecular architecture were obtained from UV-VIS spectrophotometric experiments. The studies were performed by Adsorptive Transfer Square Wave Voltammetry from the evaluation of the AO oxidation signal. The effect of the number of PEI-dsDNA bilayers (Au/MPS/(PEI-dsDNA)n) on the accumulation and electro-oxidation of AO and the influence of AO concentration on the electrochemical behavior of AO incorporated within Au/MPS/(PEI-dsDNA)1 were evaluated.