Determination of 8-hydroxy2-deoxyguanosine using electrodes modified with a dispersion of carbon nanotubes in polyethylenimine.

ALEJANDRO GUTIÉRREZ; SILVIA GUTIERREZ; GUADALUPE GARCIA; LAURA GALICIA; GUSTAVO RIVAS

ELECTROANALYSIS

WILEY-V C H VERLAG GMBH

Año: 2011 vol. 23 p. 1221 - 1228

1040-0397

Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2’-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage.  This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0107 M to 3.0105 M, with detection limit (DL) of 1.0107 M and the quantification limit (QL) of 3.0107 M.