Cytoskeletal dynamics at the leading edge of ovarian cancer cells is enhanced by LA and OL

MASNER M; BISBAL M; ACOSTA C; LUJEA N; KUNDA P

Parana

Congreso; 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2018

Argentine Society for Biochemistry and Molecular Biology

Tumoral cell migration is one of the main mechanisms of metastatic pathogenesis in ovarian cancer. We studied the effect of unsaturated Fatty Acids (FA) in vitro on migrating ovarian cancer cells and the role of the cytoskeleton on the regulation of this proccess. We used the human ovary cancer SKOV-3 cell line, in a scratch wound setting to assess different variables regarding cell migration. Low dose (16 uM) of Linoleic (LA) and Oleic fatty acids (OL) promoted migration, while Linolenic acid did not. Single cell tracking revealed that LA and OL treated cells migrate faster, more straight, polarized and orientated toward the wound closure than control. Microtubule and centromere analysis of migrating cells also showed a time dependent increase in polarization that was enhanced in the presence of LA and OL. Confocal video microscopy of EB3-Cherry transfected SKOV-3 cells exhibited an increase in microtubule +end speeds at the cell front. LifeAct-GFP SKOV-3 cells showed that FAs administration induce an increase in number, length and protrusion speeds of fillopodia at the cell front. FA treated cells featured higher lamellipodial actin dynamics with increased retrograde actin waves speed and frequency. FRAP analysis displayed an increment of actin turnover when compared with control. All these data together indicate that LA and OL modify cyostkeletal actin and tubulin protrusion dynamics at the leading edge of migrating ovarian cancer cells enhancing cell migration rate. We propose that FA composition of the peritoneal fluid could alter metastasic behaviour in ovarian cancers due to alterations in cell migration.