Role of MAP6d1 in Golgi apparatus organization and neuronal polarity

DANIELA SAUCEDO ; GONZALO QUASSOLLO; SYLVIE GORY-FAURÉ; JOSEFINA MARTIN

Buenos Aires

Congreso; FALAN Congress 2016; 2016

FALAN

Neurons are highly polarized cells typically extending several short, thick tapering dendrites and one functionally distinct long thin axon, which differ not only in their morphology but also in membrane proteins, organelles. In pyramidal neurons, Golgi apparatus has a polarized distribution that plays an important role in the development and maintenance of neuronal polarity, that is complemented by satellite Golgi outposts (GOPs) in dendrites, which play a vital role in synaptic plasticity and dendrite development. However, many questions remain about cellular mechanisms that regulate this spatial distribution. MAP6d1 is a protein which expression is highly regulated in differentiated neurons, that contains two functional domains. The amino terminal domain can interact with Golgi membranes through palmitoylation and the Mn module with the ability to interact with microtubules. In cultured neurons MAP6d1 localizes at the somatic Golgi apparatus and GOPs. We found by two hybrid screen that MAP6d1 interact with Tctex, a dynein light chain, which binds cargos to the motor complex. Here we shows that MAP6d1 alters the morphology and polarization of neuronal Golgi apparatus and GOPs in the dendritic compartment. Our observations suggest that MAP6d1 could act as a regulator of the organization and positioning of Golgi apparatus in pyramidal neurons, a phenomenon that would be of great importance to generate and maintain the dendritic arbor and neuronal polarity.