INIMEC - CONICET   05467
INSTITUTO DE INVESTIGACION MEDICA MERCEDES Y MARTIN FERREYRA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Understanding posttranslational modifications in Giardia lamblia, one of the earliest divergent eukaryotes.
Autor/es:
VRANYCH CV; MERINO C; TOUZ MC; ROPOLO AS
Lugar:
San Luis
Reunión:
Congreso; SAIB; 2011
Resumen:
Posttranslational modifications are one of the most effective ways
by which evolution has increased the versatility in protein function.
In eukaryotic cells, SUMOylation of highly conserved proteins
occurs. In the intestinal parasite G. lamblia we showed that gSentrin
protein localize mainly in the cytoplasm of Giardia trophozoites,
though also surrounding the nuclei, even inside it. By Western Blot
we observed several sumoylated proteins but, at this time, only
Arginine Deiminase (gADI) was confirmed as a sumoylated
substrate. gADI is an important enzyme in the survival and
differentiation of the parasite. During the differentiation process,
gADI translocated to the nuclei and the production of cysts was
reduced. Recently, we demonstrated that in the nuclei gADI
citrullinates histones. Moreover, when the enzyme had mutations in
the site of sumoylation, the translocation to the nuclei did not occur,
nor the citrullination of histones and the number of cysts produced
were similar to wild type trophozoites. The characterization of
SUMOylation and citrulination in Giardia are crucial to understand
the role of these posttranslational modifications in the evolution
history.
the role of these posttranslational modifications in the evolution
history.
though also surrounding the nuclei, even inside it. By Western Blot
we observed several sumoylated proteins but, at this time, only
Arginine Deiminase (gADI) was confirmed as a sumoylated
substrate. gADI is an important enzyme in the survival and
differentiation of the parasite. During the differentiation process,
gADI translocated to the nuclei and the production of cysts was
reduced. Recently, we demonstrated that in the nuclei gADI
citrullinates histones. Moreover, when the enzyme had mutations in
the site of sumoylation, the translocation to the nuclei did not occur,
nor the citrullination of histones and the number of cysts produced
were similar to wild type trophozoites. The characterization of
SUMOylation and citrulination in Giardia are crucial to understand
the role of these posttranslational modifications in the evolution
history.
the role of these posttranslational modifications in the evolution
history.
protein localize mainly in the cytoplasm of Giardia trophozoites,
though also surrounding the nuclei, even inside it. By Western Blot
we observed several sumoylated proteins but, at this time, only
Arginine Deiminase (gADI) was confirmed as a sumoylated
substrate. gADI is an important enzyme in the survival and
differentiation of the parasite. During the differentiation process,
gADI translocated to the nuclei and the production of cysts was
reduced. Recently, we demonstrated that in the nuclei gADI
citrullinates histones. Moreover, when the enzyme had mutations in
the site of sumoylation, the translocation to the nuclei did not occur,
nor the citrullination of histones and the number of cysts produced
were similar to wild type trophozoites. The characterization of
SUMOylation and citrulination in Giardia are crucial to understand
the role of these posttranslational modifications in the evolution
history.
the role of these posttranslational modifications in the evolution
history.
though also surrounding the nuclei, even inside it. By Western Blot
we observed several sumoylated proteins but, at this time, only
Arginine Deiminase (gADI) was confirmed as a sumoylated
substrate. gADI is an important enzyme in the survival and
differentiation of the parasite. During the differentiation process,
gADI translocated to the nuclei and the production of cysts was
reduced. Recently, we demonstrated that in the nuclei gADI
citrullinates histones. Moreover, when the enzyme had mutations in
the site of sumoylation, the translocation to the nuclei did not occur,
nor the citrullination of histones and the number of cysts produced
were similar to wild type trophozoites. The characterization of
SUMOylation and citrulination in Giardia are crucial to understand
the role of these posttranslational modifications in the evolution
history.
the role of these posttranslational modifications in the evolution
history.
G. lamblia we showed that gSentrin
protein localize mainly in the cytoplasm of Giardia trophozoites,
though also surrounding the nuclei, even inside it. By Western Blot
we observed several sumoylated proteins but, at this time, only
Arginine Deiminase (gADI) was confirmed as a sumoylated
substrate. gADI is an important enzyme in the survival and
differentiation of the parasite. During the differentiation process,
gADI translocated to the nuclei and the production of cysts was
reduced. Recently, we demonstrated that in the nuclei gADI
citrullinates histones. Moreover, when the enzyme had mutations in
the site of sumoylation, the translocation to the nuclei did not occur,
nor the citrullination of histones and the number of cysts produced
were similar to wild type trophozoites. The characterization of
SUMOylation and citrulination in Giardia are crucial to understand
the role of these posttranslational modifications in the evolution
history.
the role of these posttranslational modifications in the evolution
history.
though also surrounding the nuclei, even inside it. By Western Blot
we observed several sumoylated proteins but, at this time, only
Arginine Deiminase (gADI) was confirmed as a sumoylated
substrate. gADI is an important enzyme in the survival and
differentiation of the parasite. During the differentiation process,
gADI translocated to the nuclei and the production of cysts was
reduced. Recently, we demonstrated that in the nuclei gADI
citrullinates histones. Moreover, when the enzyme had mutations in
the site of sumoylation, the translocation to the nuclei did not occur,
nor the citrullination of histones and the number of cysts produced
were similar to wild type trophozoites. The characterization of
SUMOylation and citrulination in Giardia are crucial to understand
the role of these posttranslational modifications in the evolution
history.
the role of these posttranslational modifications in the evolution
history.
Giardia trophozoites,
though also surrounding the nuclei, even inside it. By Western Blot
we observed several sumoylated proteins but, at this time, only
Arginine Deiminase (gADI) was confirmed as a sumoylated
substrate. gADI is an important enzyme in the survival and
differentiation of the parasite. During the differentiation process,
gADI translocated to the nuclei and the production of cysts was
reduced. Recently, we demonstrated that in the nuclei gADI
citrullinates histones. Moreover, when the enzyme had mutations in
the site of sumoylation, the translocation to the nuclei did not occur,
nor the citrullination of histones and the number of cysts produced
were similar to wild type trophozoites. The characterization of
SUMOylation and citrulination in Giardia are crucial to understand
the role of these posttranslational modifications in the evolution
history.
the role of these posttranslational modifications in the evolution
history.
Giardia are crucial to understand
the role of these posttranslational modifications in the evolution
history.