Evidence for the involvement of Lfc and Tctex-1 in axon formation

CONDE C; ARIAS CI; ROBIN MC; LI A; SAITO M; CHUANG JZ; NAIRN A; SUNG CH; CÁCERES A

JOURNAL OF NEUROSCIENCE

SOC NEUROSCIENCE

Año: 2010 vol. 12 p. 6793 - 6800

0270-6474

RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor, localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc by inhibiting its GEF activity, decreasing Rho-GTP levels and functionally antagonizing Lfc during neurite formation.