Protective effects of an edible mushroom (Grifola gargal) on induced DNA

POSTEMSKY, PABLO; PALERMO, ANA; CURVETTO, NéSTOR

FOOD AND CHEMICAL TOXICOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2010

0278-6915

Grifola gargal is an edible polypore mushroom of Argentina and Chile with attributed antioxidant properties. Its antigenotoxicity was evaluated using the eye-SMART in Drosophila melanogaster and 7-12-dimethylbenz[á]anthracene as promutagen. Heterozygote larvae (white/white+) were grown in regular media containing 0, 12.5, 25, or 50 µmol/vial of DMBA. G. gargal fruit bodies (31 mg/mL, GgFB), mycelia from liquid culture (31 mg/mL, GgLC), mycelia grown on wheat grains (77 mg/mL, GgWG), or wheat grains (77 mg/mL, WF) were added to the larvae culture media in the absence or in the presence of 25µmol/vial DMBA. Larvae mortality in controls was 9% in water and WF containing media, and 11% in DMBA solvent, and increased up to 36% in the presence of 50 µmol/vial DMBA. GgFB, GgLC or GgWG produced a protective effect on this induced larvae mortality. Genotoxicity was not found on G. gargal and WF containing media, instead an antigenotoxic effect was found in GgFB or GgWG against 25 µmol/vial DMBA. DMBA increased LS events incidence at the 3rd instar, thus major antigenotoxic effects were detected in this larvae stage. Results reported here suggest that G. gargal is a toxic and genotoxic free mushroom and hence safe to be consumed as functional food.