Lipids droplets as an early marker of alpha-synuclein-induced neurodegeneration

ALZA, N.P.; SALVADOR, G.; SCODELARO BILBAO, P.G.; CONDE, M.

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencia; 2017

Pathological accumulation of α-synuclein (α-syn), a cytosolic proteinhighly expressed in the central nervous system, is a hallmarkof Parkinson?s disease. α-syn has the ability to bind lipids, althoughits involvement in lipid metabolism remains unknown. In previouswork, we reported that the overexpression of A53T α-syn in dopaminergicneurons increased the cellular content of neutral lipids andfatty acids (SAIB 2014-2015). The aim of the present study was tocharacterize the role of α-syn in neuronal lipid metabolism. For thispurpose, we worked with a human neuroblastoma cell line, IMR-32, that stably express the wild type form of α-syn (WT α-syn). Weobserved an increase in triacylglycerides (TAG) and cholesterolcontent in WT α-syn neurons when compared with control cells(pcDNA3) (**p