Effect of oxidative stress on adipocyte differentiation

PIWIEN-PILIPIUK, G; BLAZQUEZ, ANA; URANGA, R.; SALVADOR, G.A.

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Oxidative stress (OS) is a major characteristic of adipose tissue in obese patients and its role in adipose hyperplasia/hypertrophy has not been elucidated. The aim of our study was to analyze the effect of OS during adipogenesis as well on differentiated adipocytes. For this purpose, we worked with preadipocytes 3T3-L1 and menadione, a synthetic form of vitamin K known to generate intracellular oxygen species. Two different models were used: the ?chronic?, in which OS (5, 10 y 15 uM menadione) was present during the whole process of 3T3-L1 differentiation, and the ?acute?, in which differentiated adipocytes were exposed to OS (20 y 50 uM menadione) for 5 h.Adipogenesis, evaluated by Oil Red O staining as well as by the expression of adipogenic markers (PPARgamma, C/EBPalpha, FAS, FABP4) by Western blot, was observed to be decreased in the chronic model in a menadione concentration-dependent manner (p