INIBIBB   05455
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BAHIA BLANCA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Fluorescence STED nanoscopy reveals insights of lipid interactions with the nicotinic acetylcholine receptor
Autor/es:
C. EGGELING; D. MACHADO ANDRADE; H. TA; V. MUELLER; BORRONI M.V.; BARRANTES, F.J.
Lugar:
Buenos Aires
Reunión:
Conferencia; Synapses and dendritic spines in health and disease; 2012
Institución organizadora:
International Society for Neurochemistry
Resumen:
Fluorescence STED nanoscopy reveals insights of lipid
interactions with the nicotinic acetylcholine receptor
C. Eggeling1, D. Machado Andrade1, H. Ta1, V. Mueller1,
M. V. Borroni2, F. J. Barrantes2
1Max-Planck-Institute for biophysical Chemistry Dep.
NanoBiophotonics, Am Fassberg 11, 37077 Go¨ttingen, Germany
2Laboratory of Molecular Neurobiology, Biomedical Res. Program,
FacultyofMedicalSciences,UCA-CONICET,BuenosAires,Argentina
Lipid-protein interactions are considered to play a functional role in a
whole range of membrane-associated processes. However, their direct
and non-invasive observation in living cells is impeded by the
resolution limit of >200 nm of a conventional far-field optical
microscope. With the superior spatial resolution of STED nanoscopy
with effective focal spot sizes down to 20?30 nm in living cells, it is
now possible to directly resolve such interactions [1]. The combination
of STED nanoscopy with tools such as fluorescence correlation
spectroscopy (FCS) allows the disclosure of complex nanoscopic
dynamic processes. By performing FCS measurements in focal spots
tuned to a diameter of down to 30 nm, we have obtained new details of
molecular membrane dynamics [1]. Unlike fluorescent phosphoglycerolipids,
both fluorescent sphingolipids and certain proteins,
including the nicotinic acetylcholine receptor, are transiently
(~10 ms) trapped in cholesterol-mediated molecular complexes of
nanoscale dimensions. These interactions are distinct for different
lipids and proteins and may play an important role in cellular
functionality [2]. Here we use STED-FCS to gain new insights into the
interaction of certain phosphoglycerolipids with the nicotinic acetylcholine
receptor in the plasma membrane of living cells.
References
[1] C Eggeling? Nature (2009), 457, 1159; C Ringemann? New
J Physics (2009) 11, 103054.
[2] V Mueller? Biophys J (2011) 101, 1651.