Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity

CHAN FRANCIS KA-MING; MORIWAKI KENTA; DE ROSA MARIA JOSE

Methods in Molecular Biology, Protein Blotting and Detection

Humana Press

Lugar: New York; Año: 2011;

Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrisis. A key signature for necrotic cells is the permeabilization of plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate based colorimetric absorbance assay.