INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The Trk/BDNF cell signaling pathway participates in the neural crest specification
Autor/es:
GARAY MERCEDES GUADALUPE; AYBAR MANUEL JAVIER; VEGA-LOPEZ GUILLERMO
Lugar:
Buenos Aires
Reunión:
Congreso; Xth Meeting of the Latin American Society for Developmental Biology; 2019
Institución organizadora:
Latin American Society for Developmental Biology
Resumen:
The neural crest (NC) is a migratory multipotent cell population, exclusive of vertebrate embryos, that defined the evolution of this filum. The NC gives rise to a wide variety of cell types and tissues such as sensitive neurons and glia, neuroendocrine cells, connective tissue and cartilage of the head, pigmentary cells, among others. The NC cells arise in the ectoderm between the neural plate and prospective epidermis, undergo the ephitelial-to-mesenchymal transition and then migrate through the entire embryo to reach their final destination. In our laboratory we have demonstrated that multiple cellular signaling pathway participate in NC formation, migration and specification during Xenopus laevis early embryogenesis. The Trk/BDNF cell signaling pathway has been implicated in many cellular processes of neural precursors selection and in the development of some NC derivatives in chicken and mouse. We have analyzed the spatiotemporal expression pattern of the components of this signaling pathway in the amphibian Xenopus laevis. The gain of function analysis showed that changes in the expression or function of trk and bdnf affected the expression of specifics neural crest markers and NC specification. Furthermore, the use of dominant negative of Trk receptor and specific knock down of the trk mRNA by the use of a morpholino antisense oligonucleotide produce the opposite effect. To summarize, we have found that the Trk/BDNF signaling is essential for NC formation.