LOW COST PAPER-BASED (BIO)ELECTROANALYTICAL DEVICES

LUCÍA BLANCO-COVIÁN; ESTEFANÍA NUÑEZ-BAJO; M. TERESA FERNANDEZ-ABEDUL; ANDREA GONZALEZ-LOPEZ; PAULA I. NANNI; ROSSANA E. MADRID; OLAYA AMOR-GUTIERREZ; ESTEFANÍA COSTA-RAMA; CARMEN BLANCO-LOPEZ

Oviedo

Workshop; 2nd Workshop on Electrochemistry devices and 2nd Workshop Electrobionet; 2017

Nowadays, miniaturization and reduction of the costs of analytical devices,especially in diagnostic applications, are fields in continuous growth. Portable andeasy-to-use devices are produced to reach remote areas where untrainedpersonnel are in charge of the analysis. Electrochemical detection fits properlywith these purposes especially when combined with low-cost materials. In thiswork, we are presenting different devices based on paper and conductive filmsor common mass-produced materials such as: pins or staples.Firstly, we have designed some bioanalytical devices based on enzymaticreactions. The first one is a maskless biosensor for glucose that useschromatographic paper and carbon ink as working electrode with gold-plated lowcostconnector headers as reference and auxiliary electrodes. [1]. The secondapproach uses stainless steel pins as electrodes, with this used as workingelectrode coated with carbon ink. Hydrophobic paper [2] or transparency film canbe used as support [3].On the other hand, immunoassays were coupled to a staple-basedelectrochemical detector for allowing quantitative analysis of human tissuetransglutaminase (related to celiac disease) on paper. Secondly, we havedeveloped a novel flow-through assay for pneumolysin. In this case, paper isspotted with capture reagents. After interaction with the target, the intensity of thecapture spot can be quantified by coupling metallic thin-film electrodes.Finally, paper is not only quite useful for biosensors development, also fordesigning lab-on-paper devices. In this work, we are also presenting a paperbaseddevice with integrated sample injection, electrophoretic separation andelectrochemical detection.