Inorganic phosphate modulates biofilm formation and stress resistance in S. tiphimurium

OVEJERO, SILVIA N; GRILLO PUERTAS, MARIANA; RAPISARDA, VIVIANA ANDREA; RINTOUL, MARIA REGINA

Mendoza

Congreso; XLVIII Reunión Anual SAIB; 2012

INORGANIC PHOSPHATE MODULATES BIOFILMFORMATION AND STRESS RESISTANCE IN S.Ovejero SN, Grillo-Puertas M, RapisardaVA, .Inst de Química Biológica ? INSIBIO, CONICET-UNT. San Miguelde Tucumán, Argentina. E-mail: grintoul@fbqf.unt.edu.arE. coliS. thyphimuriumS. thyphimurimthyphimuriumRintoulMRIn several organisms, inorganic polyphosphate (polyP) formationwas shown to be critical for attributes such as motility, quorumsensing, biofilm formation, resistance to stress, and stationaryphasesurvival.We have previously reported that, stationarycells grown in media containing at least 37 mM phosphate (Pi)maintain high polyP levels and high viability. They also exhibitresistance toH O and low capacity to form biofilm. Here, the effectof different Pi concentrations on cell viability, polyP level, oxidativestress resistance and biofilm formation capacity (BFC) wasevaluated througout the growth curve of .Viability,oxidative stress resistance and polyP levels were high in stationaryphase cells grown in high Pi concentration media (=25 mM).Biofilm formation in polystyrene, glass, polypropylene andstainless steel were evaluated in static condition at 30ºC withO?Toole and Kolter method. A preference for glass orpolypropylene surfaces was observed. At 24 h, BFC was dependenton the Pi concentration of the planktonic preinocula and of theincubation media. BFC was maximal at 20mMPi. According to ourresults, we conclude that Pi concentration is an important signal forbiofilm formation and stationary phase fitness incells.