Pathogen-Induced Accumulation of an Ellagitannin Elicits the Plant Defense Response

MAMANí, ALICIA; FILLIPONNE, PAULA; GRELLET, CARLOS; WELIN, BJORN; CASTAGNARO, ATILIO; DIAZ RICCI, JUAN CARLOS

MOLECULAR PLANT-MICROBE INTERACTIONS

AMER PHYTOPATHOLOGICAL SOC

Año: 2012 vol. 25 p. 1430 - 1439

0894-0282

In an incompatible interaction between Colletotrichum fragariae and strawberry plants, the accumulation of phenolic compounds in plant leaves was observed. A particularly abundant penta-esterified ellagitannin that accumulated in response to pathogen attack was identified as 1-0-galloyl-2,3;4,6-bis-hexahydroxydiphenoyl-B-D-glucopyranose (HeT) by mass spectroscopy and nuclear magnetic resonance. Foliar application of purified HeT prior to inoculation with a virulent pathogen was shown to increase resistance toward C. acutatum in strawberry plants and to Xanthomonas citri subsp. citri in lemon plants. The induced resistance in strawberry was associated with a rapid oxidative burst, callose deposition, a transient increase of salicylic acid in phloem, and induction of gene expression responsive to salicylic acid. Results obtained suggested that HeT could be a common plant defense response molecule capable of inducing pathogen resistance in different plant species