INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Genetic diversity assessment and genotype identification in sugarcane based on DNA markers and morphological traits
Autor/es:
M. F. PERERA M. E. ARIAS D. COSTILLA A. C. LUQUE M. B. GARCÝ´A C. DÝ´AZ ROMERO J. RACEDO S. OSTENGO M. P. FILIPPONE M. I. CUENYA A. P. CASTAGNARO
Revista:
EUPHYTICA
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2012 p. 1 - 22
ISSN:
0014-2336
Resumen:
Abstract Sugarcane is known for its highly complex
genetics and more knowledge is needed for better use
and conservation of genetic materials. In order to
identify genotypes and to assess genetic diversity,
diverse data sets such as morphological and molecular
markers are used as a general approach. To evaluate the
usefulness of different markers, important sugarcane
genotypes in Argentina were characterized by AFLP,
SSR and morphological traits. All genotypes characterized
were grouped in one main cluster in dendrograms
using two independent softwares. Interestingly,
local genotypes grouped together with USA varieties
and no clear genetic differentiation could be found
probably due to intensive germplasm exchange
between these breeding programs. The molecular
markers tested were useful for genetic diversity
assessment as well as for genotype identification.
These markers should be included in the internationally
established characters for sugarcane variety protection
as they give a better view on whole genome
complexity. Additionally, genetic similarities obtained
from molecular markers will provide more accurate
information to breeders than the pedigree method,
especially when considering the asymmetric genetic
inheritance of sugarcane. Morphological traits are
valuable tools to identify genotypes since they reflect
external resemblance more than genetic relatedness.
When they were combined with molecular markers the
dendogram obtained revealed genetic relationships
and the genetic diversity was better estimated. In
summary, both methods appear to be useful, complementing
each other and should be used together to
assist sugarcane breeders in estimating genetic diversity,
electing parents for crossings, identifying superior
lines and to protect intellectual property rights.
genetics and more knowledge is needed for better use
and conservation of genetic materials. In order to
identify genotypes and to assess genetic diversity,
diverse data sets such as morphological and molecular
markers are used as a general approach. To evaluate the
usefulness of different markers, important sugarcane
genotypes in Argentina were characterized by AFLP,
SSR and morphological traits. All genotypes characterized
were grouped in one main cluster in dendrograms
using two independent softwares. Interestingly,
local genotypes grouped together with USA varieties
and no clear genetic differentiation could be found
probably due to intensive germplasm exchange
between these breeding programs. The molecular
markers tested were useful for genetic diversity
assessment as well as for genotype identification.
These markers should be included in the internationally
established characters for sugarcane variety protection
as they give a better view on whole genome
complexity. Additionally, genetic similarities obtained
from molecular markers will provide more accurate
information to breeders than the pedigree method,
especially when considering the asymmetric genetic
inheritance of sugarcane. Morphological traits are
valuable tools to identify genotypes since they reflect
external resemblance more than genetic relatedness.
When they were combined with molecular markers the
dendogram obtained revealed genetic relationships
and the genetic diversity was better estimated. In
summary, both methods appear to be useful, complementing
each other and should be used together to
assist sugarcane breeders in estimating genetic diversity,
electing parents for crossings, identifying superior
lines and to protect intellectual property rights.
genetics and more knowledge is needed for better use
and conservation of genetic materials. In order to
identify genotypes and to assess genetic diversity,
diverse data sets such as morphological and molecular
markers are used as a general approach. To evaluate the
usefulness of different markers, important sugarcane
genotypes in Argentina were characterized by AFLP,
SSR and morphological traits. All genotypes characterized
were grouped in one main cluster in dendrograms
using two independent softwares. Interestingly,
local genotypes grouped together with USA varieties
and no clear genetic differentiation could be found
probably due to intensive germplasm exchange
between these breeding programs. The molecular
markers tested were useful for genetic diversity
assessment as well as for genotype identification.
These markers should be included in the internationally
established characters for sugarcane variety protection
as they give a better view on whole genome
complexity. Additionally, genetic similarities obtained
from molecular markers will provide more accurate
information to breeders than the pedigree method,
especially when considering the asymmetric genetic
inheritance of sugarcane. Morphological traits are
valuable tools to identify genotypes since they reflect
external resemblance more than genetic relatedness.
When they were combined with molecular markers the
dendogram obtained revealed genetic relationships
and the genetic diversity was better estimated. In
summary, both methods appear to be useful, complementing
each other and should be used together to
assist sugarcane breeders in estimating genetic diversity,
electing parents for crossings, identifying superior
lines and to protect intellectual property rights.
genetics and more knowledge is needed for better use
and conservation of genetic materials. In order to
identify genotypes and to assess genetic diversity,
diverse data sets such as morphological and molecular
markers are used as a general approach. To evaluate the
usefulness of different markers, important sugarcane
genotypes in Argentina were characterized by AFLP,
SSR and morphological traits. All genotypes characterized
were grouped in one main cluster in dendrograms
using two independent softwares. Interestingly,
local genotypes grouped together with USA varieties
and no clear genetic differentiation could be found
probably due to intensive germplasm exchange
between these breeding programs. The molecular
markers tested were useful for genetic diversity
assessment as well as for genotype identification.
These markers should be included in the internationally
established characters for sugarcane variety protection
as they give a better view on whole genome
complexity. Additionally, genetic similarities obtained
from molecular markers will provide more accurate
information to breeders than the pedigree method,
especially when considering the asymmetric genetic
inheritance of sugarcane. Morphological traits are
valuable tools to identify genotypes since they reflect
external resemblance more than genetic relatedness.
When they were combined with molecular markers the
dendogram obtained revealed genetic relationships
and the genetic diversity was better estimated. In
summary, both methods appear to be useful, complementing
each other and should be used together to
assist sugarcane breeders in estimating genetic diversity,
electing parents for crossings, identifying superior
lines and to protect intellectual property rights.
Sugarcane is known for its highly complex
genetics and more knowledge is needed for better use
and conservation of genetic materials. In order to
identify genotypes and to assess genetic diversity,
diverse data sets such as morphological and molecular
markers are used as a general approach. To evaluate the
usefulness of different markers, important sugarcane
genotypes in Argentina were characterized by AFLP,
SSR and morphological traits. All genotypes characterized
were grouped in one main cluster in dendrograms
using two independent softwares. Interestingly,
local genotypes grouped together with USA varieties
and no clear genetic differentiation could be found
probably due to intensive germplasm exchange
between these breeding programs. The molecular
markers tested were useful for genetic diversity
assessment as well as for genotype identification.
These markers should be included in the internationally
established characters for sugarcane variety protection
as they give a better view on whole genome
complexity. Additionally, genetic similarities obtained
from molecular markers will provide more accurate
information to breeders than the pedigree method,
especially when considering the asymmetric genetic
inheritance of sugarcane. Morphological traits are
valuable tools to identify genotypes since they reflect
external resemblance more than genetic relatedness.
When they were combined with molecular markers the
dendogram obtained revealed genetic relationships
and the genetic diversity was better estimated. In
summary, both methods appear to be useful, complementing
each other and should be used together to
assist sugarcane breeders in estimating genetic diversity,
electing parents for crossings, identifying superior
lines and to protect intellectual property rights.