Differential response of cultured human cells exponed to copper overload and natural polyphenols.

ARNAL, NATHALIE; MARÍA J.T. DE ALANIZ; CARLOS A. MARRA

San Miguel de Tucumán. Argentina

Congreso; XLV Reunión anual de la sociedad argentina de Investigación en Bioquímica y Biología Molecular.; 2009

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

The overload of copper (Cu) induces a failure in the antioxidant defence system. We studied the effect caused by the association of Cu with natural polyphenols. HepG2 (liver) and A549 (lung) derived human cells were treated with Cu 100 ìM/24-48 h and simultaneous or differed supplementation with 100cafeic acid (CA), resveratrol (RES), or curcumin (CR) (100 ìM). All the treatments reduced the total cellular protein and the cellular viability, except for the CR-treated cells. Cells were homogeinized and analyzed for nitrates + nitrites, TBARS, and protein carbonyls as biomarkers for oxidative stress. All the parameters were elevated with the time of treatment under Cu supplementation, being more pronounced in HepG2 than A549. Association with CA or RES produced an increment in the level of damage observed on both type of cells. Again, the degree of damage was more evident in HepG2. In contrast, inclusion of CR ameliorates the oxidative stress condition induced by Cu, especially in A549. Glutathione was increased only In HepG2 in response to the damages evoked by the association of Cu with CA or RES. Results suggest that it should be crucial to consider the chemical structure of polyphenols as antioxidants to prevent the damages associated to Cu overload. CR might be protective, while RES or CA could be pro-oxidants in association with non-physiological Cu levels.