INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Analysis of Mammalian Soluble Lipid Binding Proteins
Autor/es:
FALOMIR LOCKHART LJ; FRANCHINI G; DEGERÓNIMO E; RODRIGUEZ SAWICKI L; BOTTASSO, NATALIA; CÓRSICO B
Lugar:
Bs As
Reunión:
Jornada; Jornadas de la Academis Nacional de Farmacia y Bioquímica; 2009
Institución organizadora:
Academis Nacional de Farmacia y Bioquímica
Resumen:
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Functional Analysis
of Mammalian Soluble Lipid Binding Proteins
Falomir Lockhart, LJ; Franchini GR; De Gerónimo E;
Guerbi, MX; Rodriguez Sawicki, L; Bottasso, N; and Córsico B
Instituto de
Investigaciones Bioquímicas de La Plata (INIBIOLP) (CONICET-UNLP), Fac. de Cs. Médicas, Calle 60 y 120 (1900) La Plata
The evolution of different families of intracellular
soluble lipid binding proteins (SLBP) may be connected to the wide range of
functions attributed to lipids as well as their low solubility in the cellular
media. Among these SLBP, the mammalian fatty acid binding proteins (FABP) are
ubiquitously produced. The large number of FABP types and the distinct
expression pattern of each of them suggest overlapping as well as distinct
functions in specific tissues; based on specific structural elements.
Structure-function studies indicate that subtle conformational changes that
occur upon ligand binding may promote distinct FABP-protein or FABP-membrane
interactions that could define their specificity.
We used a combination of in vitro and in cell studies
to assess the differential functionality of abundantly coexpressed FABP in the
enterocyte. First, the use of several structural variants highlighted the
importance of the helical region and specific residues have been identified in
the Portal Domain, crucial for ligand transport and membrane binding properties
of FABP. On the other hand, modification of their expression in culture cells has
an impact on several cellular processes, mainly lipid metabolism but also cell
proliferation and differentiation. FABPs participation in regulating fatty
acid metabolism, may ultimately impact on systemic metabolism.