Anticancer activity of lipidic nanoparticles containing essential oils components in hepatocarcinoma cells

B RODENAK KLADNIEW; M GARCÍA DE BRAVO; G ISLÁN; N DURÁN; N NOACCO; GR CASTRO

Vellore

Conferencia; International Conference on Current Trends in Biotechnology (ICCB 2016); 2016

School of Bio Sciences and Technology VIT University

Human hepatocellular carcinoma (HCC) is one of the most common and lethal cancers worldwide and current therapies are often limited. New alternatives are focus in the use of plant-derived compounds with anticancer activities. To improve their bioavailability, solid lipid nanoparticles (SLN) arise as a potent tool for improving their efficacy in physiological environments. In the present work, linalool (LN) -a plant-derived monoterpene- was loaded into SLNs and the anticancer potential was evaluated in HCC HepG2 cells. SLNs composed of myristyl myristate were synthesized by ultrasonication method and LN encapsulation efficiency around 90% was obtained. An in vitro controlled released was observed for 72 h. The nanoparticles exhibited spherical size and a mean diameter of 92.3 ±0.7 nm with low dispersion (PDI 0.21) determined by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The loaded nanoparticles were extensively characterized by FTIR, XRD, DSC and TGA, indicating a clear nanostructuration of the lipidic matrix after LN incorporation. The formulation resulted cytotoxic against HepG2 cells in a dose/time manner, enhancing the anticancer activity of free LN. In addition, the cellular uptake of fluorescent-labeled SLN revealed the ability of nanoparticles to enter into cells and intracellularly deliver the cargo molecules.