INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Synthesis and cell internalization of magnetite nanoparticles for magnetic hyperthermia applications
Autor/es:
DE SOUSA E; GIRARDIN P; FERNANDEZ VAN RAAP MB; PASQUEVICH G; MENDOZA ZELIS P; BRUVERA A; SANCHEZ F; GOYA RG; SOSA YE
Lugar:
La Plata
Reunión:
Conferencia; 2011 Humboldt Kolleg International Conference on Physics; 2011
Institución organizadora:
UNLP Dept of Physics
Resumen:
Magnetic hyperthermia treatment promises to be a viable alternative or
complementary procedure for localized cancerous tumors therapy. The treatment
consists of locally injecting magnetic nanoparticles (MNP) into the tumor and exposing
it to an oscillating magnetic field in the radio frequency range. When exposed to this
field, the MNP dissipates energy causing a temperature increase in the zone that
contains nanoparticles, inducing tumor cell death by apoptosis at or above 42 °C. MNP
energy dissipation is characterized by the specific power loss parameter (SLP) which
expresses the power absorbed by the MNP from the field per mass unit of MNP.
13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g,
were synthesized by co-precipitation route using iron salts and a strong base, and finally
set into aqueous suspension. Their physical properties were characterized with various
techniques and suggest their adequacy for hyperthermia treatment and cell
internalization requirements. Measured SPL value for this solution, using a coil fed with
a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1.
Internalization methodology for anionic magnetic nanoparticles, which is
achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is
known to be well established in various tumoural and normal cells. Here, bare MNP
nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for
two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows theMNP
energy dissipation is characterized by the specific power loss parameter (SLP) which
expresses the power absorbed by the MNP from the field per mass unit of MNP.
13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g,
were synthesized by co-precipitation route using iron salts and a strong base, and finally
set into aqueous suspension. Their physical properties were characterized with various
techniques and suggest their adequacy for hyperthermia treatment and cell
internalization requirements. Measured SPL value for this solution, using a coil fed with
a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1.
Internalization methodology for anionic magnetic nanoparticles, which is
achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is
known to be well established in various tumoural and normal cells. Here, bare MNP
nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for
two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows theloss parameter (SLP) which
expresses the power absorbed by the MNP from the field per mass unit of MNP.
13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g,
were synthesized by co-precipitation route using iron salts and a strong base, and finally
set into aqueous suspension. Their physical properties were characterized with various
techniques and suggest their adequacy for hyperthermia treatment and cell
internalization requirements. Measured SPL value for this solution, using a coil fed with
a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1.
Internalization methodology for anionic magnetic nanoparticles, which is
achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is
known to be well established in various tumoural and normal cells. Here, bare MNP
nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for
two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows theMNP from the field per mass unit of MNP.
13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g,
were synthesized by co-precipitation route using iron salts and a strong base, and finally
set into aqueous suspension. Their physical properties were characterized with various
techniques and suggest their adequacy for hyperthermia treatment and cell
internalization requirements. Measured SPL value for this solution, using a coil fed with
a signal of 260 kHz and amplitude of 500 Oe (39.8 kA/m), was 203 W g-1.
Internalization methodology for anionic magnetic nanoparticles, which is
achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is
known to be well established in various tumoural and normal cells. Here, bare MNP
nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for
two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows the-1.
Internalization methodology for anionic magnetic nanoparticles, which is
achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is
known to be well established in various tumoural and normal cells. Here, bare MNP
nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for
two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows thefrom human lung adenocarcinoma for
two NP concentrations of 20 and 50 ìg/ml. MNP incorporation into cells follows thels follows the
endocytic pathway. It is observed that MNP concentration inside cells is larger and
more densely packed for the larger MNP concentration. Confocal, atomic force and
transmission electron microscopies were used to image the in vitro uptake of
nanoparticles by A549 cells.s is larger and
more densely packed for the larger MNP concentration. Confocal, atomic force and
transmission electron microscopies were used to image the in vitro uptake of
nanoparticles by A549 cells.