Intestinal Fatty Acid Binding Proteins: Importance of Alpha-I Helix in Fatty Acid Mechanism of Transfer to Phospholipids Membranes.

FRANCHINI, G.R.; STORCH, J; CÓRSICO, BETINA

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Elsevier

Lugar: Amsterdam; Año: 2008 vol. 1781 p. 192 - 199

1388-1981

Intestinal FABP (IFABP) and liver FABP (LFABP), homologous proteins expressed at high levels in intestinal absorptive cells, employ markedly different mechanisms of fatty acid transfer to acceptor model membranes. Transfer from IFABP occurs during protein–membrane collisional interactions, while for LFABP transfer occurs by diffusion through the aqueous phase. In addition, transfer from IFABP is markedly faster than from LFABP. The overall goal of this study was to further explore the structural differences between IFABP and LFABP which underlie their large functional differences in ligand transport. In particular, we addressed the role of the áI-helix domain in the unique transport properties of intestinal FABP. A chimeric protein was engineered with the ‘body’ (ligand binding domain) of IFABP and the áI-helix of LFABP (á(I) LâIFABP), and the fatty acid transfer properties of the chimeric FABP were examined using a fluorescence resonance energy transfer assay. The results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane LâIFABP), and the fatty acid transfer properties of the chimeric FABP were examined using a fluorescence resonance energy transfer assay. The results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane LâIFABP), and the fatty acid transfer properties of the chimeric FABP were examined using a fluorescence resonance energy transfer assay. The results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane LâIFABP), and the fatty acid transfer properties of the chimeric FABP were examined using a fluorescence resonance energy transfer assay. The results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane of intestinal FABP. A chimeric protein was engineered with the ‘body’ (ligand binding domain) of IFABP and the áI-helix of LFABP (á(I) LâIFABP), and the fatty acid transfer properties of the chimeric FABP were examined using a fluorescence resonance energy transfer assay. The results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane LâIFABP), and the fatty acid transfer properties of the chimeric FABP were examined using a fluorescence resonance energy transfer assay. The results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane results showed a significant decrease in the absolute rate of FA transfer from á(I)LâIFABP compared to IFABP. The results indicate that the áIhelix is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-helix in the formation of a protein–membrane is crucial for IFABP collisional FA transfer, and further indicate the participation of the áII-