INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Role of thymulin on the somatotropic axis in vivo
Autor/es:
REGGIANI PC; MARTINES E; CAMIHORT GA; POCH B; GOYA RG; CONSOLE GM
Revista:
LIFE SCIENCES
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 91 p. 166 - 171
ISSN:
0024-3205
Resumen:
Aims: There is clear evidence for the existence of a bi-directional thymussomatotropic axis and several studies sug- 22
gest that the thymic peptide thymulinmay be involved in this communication.We undertook to assess the impact 23
of serum thymulin immunoneutralization in C57BL/6 mice and that of neonatal thymulin gene therapy (NTGT) in 24
nude mice on body weight (BW) gain and on the histomorphometric profile of the somatotrope population. 25
Mainmethods: Immunoneutralization of thymulinwas done frompostnatal day 1 to 35 by i.p. injections of rab- 26
bit anti-thymulin serum(α-FTS) and normal rabbit serum(NRS) in controls. NTGTwas implemented in nudes 27
using an adenoviral vector expressing a synthetic gene for thymulin (RAd-FTS). On postnatal day 1, heterozy- 28
gous (nu/+) and homozygous (nu/nu) pups received a single bilateral i.m. injection either RAd-FTS or 29
RAd-GFP (a control vector expressing green fluorescent protein). BW gain was recorded and at the end of 30
the study the pituitarieswere immunostained for growth hormone (GH). SerumGH and thymulinwere deter- 31
mined by radioimmunoassay and bioassay, respectively. 32
Key findings: Thymulin immunoneutralization induced a significant decrease in BW gain, serum GH and 33
somatotrope cell density as well as an increase in somatotrope cell size. NTGT markedly increased BW gain, 34
serum thymulin (Pb0.01) and somatotrope cell and volume density in nu/nu mice. 35
Significance: Our results suggest that thymulin plays a relevant physiological role on the thymussomatotropic 36
axis in mice. 37
© 2012 Published by Elsevier