INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
artículos
Título:
Dietary lipid-induced changes in enzymes of hepatic lipid metabolism.
Autor/es:
HURTADO DE CATALFO, GRACIELA E.; ALANIZ, MARÍA J.T. DE; MARRA, CARLOS ALBERTO
Revista:
NUTRITION
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Amsterdam; Año: 2012
ISSN:
0899-9007
Resumen:
Objective:
To
investigate the effect of different dietary oils on the main hepatic enzymes
involved in metabolism and their impact
on the oxidative stress status.
Methods: Twenty four male
Wistar rats were fed for 60 d on the same basal diet plus different lipid
sources as commercial oils: soybean-S, olive-O, coconut-C and grape-seed-G.
After sacrifice, liver lipid fatty acid composition, enzymatic and
non-enzymatic components of the antioxidant defense system and the activity of
enzymes involved in lipid metabolism were determined. The concentration of [Ca++]
in plasma and liver homogenates was also measured.
Results: The diets produced
significant changes in total and polar lipid fatty acid composition together
with alterations on the key enzyme activities involved in lipid metabolism. S-
and G-groups increased significantly the oxidative stress biomarkers. Enzymatic
and non enzymatic components of the antioxidant defense system were elevated in
O- and C-groups. The highest levels of nitrite plus nitrate [NOx] were observed
in S- and G-groups compared with O and C in plasma and in liver homogenates.
They were directly correlated with [Ca++] concentration. The most beneficial
effects were obtained with olive oil. However, it was necessary to study in more
detail appropriate mixtures of olive and soybean oils to provide adequate
balance between n-3 and n-6 fatty acids.
Conclusion: Different dietary
oils modify lipid composition of plasma and liver, local and systemic
antioxidant status, and the activity of key enzymes of lipid metabolism. The
interrelationship between [Ca++] and [NOx] could be the causal factor underlying the
observed changes.