Lectin-modified surfaces for the real-time determination of Bone Alkaline Phosphatase by Surface Plasmon Resonance (SPR) Spectroscopy

AZZARONI, OMAR; MADRID, ROSSANA E.; ESTEBAN PICCININI; SAPPIA, LUCIANO D.; MADRID, ROSSANA E.; MARMISOLLÉ, WALDEMAR; SAPPIA, LUCIANO D.; MARMISOLLÉ, WALDEMAR; AZZARONI, OMAR; ESTEBAN PICCININI

Buenos Aires

Workshop; 1st Argentine-German Workshop on Nanotechnology and Nanobiosensors; 2017

Instituto Nacional de Tecnología Industrial

The biological interaction of lectins and carbohydrates rules many recognitions processes found in living organisms [1]. These interactions can be exploited to develop highly stable lectin and glycoprotein biointerfaces [2,3]. In this work, we constructed carbohydrate layers on gold substrates for the immobilization of the lectin wheat germ agglutinin (WGA). The WGA-modified surfaces were used as arecognition element of glycosylated proteins present in human serum. The lectin-glycoprotein recognition event was evaluated by Resonance Plasmon Spectroscopy (SPR), which has a resolution in the subnanometric scale [4].