Development and evaluation of a SPR-based immunosensor for the diagnosis of Cutaneous Leishmaniasis

DAZA MILLONE, M. A.; VELA, M. E.; CHAIN, C. Y.; KUBOTA, L. T.; PIRES SOUTO, D. E.; MONTEIRO DE ANDRADE, H.

Riva del Garda

Congreso; 5th International Conference on Bio-Sensing Technology; 2017

Protozoan Leishmania is the etiological agent of Cutaneous Leishmaniasis (CL) disease, which needs urgent progress in its immunological diagnosis due to the increase in the number of cases in the last decades. Direct exams or culture should be the method of choice to confirm a clinical suspect of CL disease, because they are reliable. However, these tests are time consuming and labor intensive and have low sensitivity. Consequently, we need to search for other, complementary, methods of diagnosis to be employed in outbreaks, or to carry out epidemiological studies to analyze a large number of patients at the same time and give a faster diagnosis. In this context, label free immunosensors based on surface plasmon resonance (SPR) open up broad prospects for the development of new methodologies in clinical diagnosis. In recent years, several authors have described the development of such systems for the immunodetection of various diseases, including dengue virus [1], Chagas disease [2] and visceral leishmaniasis [3]. However, at present, SPR-based immunosensors have not been explored for the diagnosis of Cutaneous Leishmaniasis disease. [1] Surface plasmon resonance based immunosensor for serological diagnosis of dengue virus infection. S. Kumbhata, , K. Sharmaa, R. Gehlota, A. Solankib, V. Joshic. Journal of Pharmaceutical and Biomedical Analysis Volume 52, Issue 2, 5 June 2010, Pages 255?259.[2] Development and evaluation of a SPR-based immunosensor for detection of anti-Trypanosoma cruzi antibodies in human serum. J. G.G. Luz, D. E.P. Souto, G. F. Machado-Assis, M. de Lana, L. T. Kubota, R. C.S. Luz, F. S. Damos, H. R. Martins. Sensors and Actuators B 212 (2015) 287?296[3] Development of a label-free immunosensor based on surface plasmon resonance technique for the detection of anti-Leishmania infantum antibodies in canine serum. D. E. P Souto, J. V. Silva, H. R. Martins, A. B. Reis, R. C. S. Luz, L. T. Kubota. F. S. Damos. Biosensors and Bioelectronics 46 (2013) 22?29.