Evaluation of cell damage produced by Fe ions released as degradation products of biomaterials. Influence of pH changes

FAGALI NS; GRILLO CA; PUNTARULO S; FERNÁNDEZ LORENZO MA

Maratea

Simposio; 6th Biometal. Symiposium on Biodegradable Metals for Biomedical Applications; 2014

Fe and some of its alloys have been proposed for the manufacture of temporary stents because they are susceptible to a fast corrosion in biological media [1]. However, the release of ions and particles during dissolution may cause side effects like oxidative stress and inflammation in the tissues in contact with the implant. Despite its importance the mechanisms by which these deleterious effects are produced has not been sufficiently described in the literature. In this paper, the effect of Fe2+ and Fe3+ ions and pH changes on cultures of CHO-K1 cells was evaluated. Results indicate that cytotoxicity depends on Fe ions concentration and pH changes derived from Fe dissolution while oxidative stress depends only to Fe concentration. These changes occurring at the interface of biomaterial/biological medium during the degradation of Fe may markedly affect the viability of cells surrounding the iron-based implant.