Studying the binding kinetics of an antigen- antibody model system by Surface Plasmon Resonance: towards the development of a biosensor

CECILIA Y. CHAIN; MARÍA A. DAZA MILLONE; ALEJANDRO RAMIREZ; MARTÍN RUMBO; GUILLERMO DOCENA; MARÍA E. VELA

Encuentro; IMCS 2014- The 15th International Meeting on Chemical Sensors; 2014

We have studied the casein-anti-casein binding kinetics using two different strategies in an SPR commercial device: i) casein was immobilized onto the solid phase and assessed with a mouse monoclonal antibody specific to bovine casein at different concentrations in solution and ii) the solid substrate was coated with the anti-casein monoclonal antibody and the binding to different concentrations of soluble casein was assessed.