Effect of Mg particles on MCTT·-E1 and J774 cellular cycle. Influence of fluoride treatment

ALVAREZ, F; LOZANO PUERTO, R; PEREZ MACEDA, B; GRILLO, C; FERNÁNDEZ LORENZO DE MELE, M

Simposio; 6th Biometal. Symiposium on Biodegradable Metals for Biomedical Applications; 2014

Mg-based materials are promising for orthopedic, dental, and cardiovascular applications [1]. After Mg implantation its high degradation rate causes the release of microscopic debris particles (MgP) and metal ions. Protective treatments involving the generation of magnesium fluoride conversion coatings (Mg-F-C) have been proposed [2]. The aim of this work was to evaluate the possible cytotoxic effects of MgP, and with and without F-C on macrophage (J774) and osteoblast (MC3T3-E1) cell lines. Mg-F-C temporally decrease corrosion rate of Mg [2]. Reduction of corrosion rate implies the decrease in the release of ions and may increase biocompatibility. Notwithstanding this, in a previous report with J774 cells, higher effect on LDH assays was detected for MgP01 than in case of MgP for ≥1000 µg/ml. Coincidently, present results showed that Mg-F-C affect viability of both cell lines assayed (MC3T3-E1 and J774). Macrophages seem to be more sensitive to MgP, MgP01 and MgP02 (1000 µg/ml) than MC3T3-E1. The presence of a Mg-F-C on MgP01 and MgP02 also affects the phases of the J774 cell cycle severally.