Nanobiocomposite based on natural polyelectrolytes for bone regeneration

M. SILVINA MOLINUEVO; M. SOLEDAD BELLUZO; M. SUSANA CORTIZO; LARA F. MEDINA; ANA M. CORTIZO

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

WILEY-LISS, DIV JOHN WILEY & SONS INC

Lugar: New York; Año: 2020 vol. 108 p. 1 - 12

1549-3296

We developed a composite hydrogel based on chitosan and carboxymethyl cellulosewith nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration. The 3D porous-structure of the biocomposite scaffolds were confirmed by Scanning Electron Microscopy andMicrotomography analysis. Infrared analysis did not show specific interactionsbetween the organic components of the composite and nHA in the scaffold. Thehydrogel properties of the matrices were studied by swelling and mechanical tests, indicating that the scaffold presented a good mechanical behavior. The degradation test demonstrated that the material is slowly degraded, while the addition of nHA slightly influences the degradation of the scaffolds. Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.On the other hand, no cytotoxic effect was observed when RAW264.7 cells wereseeded on the scaffolds. Altogether, our results allow us to conclude that thesenanobiocomposites are promising candidates to induce bone tissue regeneration.