Trypanosoma cruzi virulence factors for the diagnosis of Chagas' disease

CHAIN, CECILIA YAMIL; LABRIOLA, CARLOS A.; CISNEROS, JOSÉ SEBASTIÁN; KUBOTA, LAURO T.; CHAIN, CECILIA YAMIL; LABRIOLA, CARLOS A.; CISNEROS, JOSÉ SEBASTIÁN; KUBOTA, LAURO T.; SOUTO, DÊNIO EMANUEL PIRES; MILLONE, MARÍA ANTONIETA DAZA; LOPEZ-ALBIZU, CONSTANZA; RUIZ, ANDRÉS MARIANO; SOUTO, DÊNIO EMANUEL PIRES; MILLONE, MARÍA ANTONIETA DAZA; LOPEZ-ALBIZU, CONSTANZA; RUIZ, ANDRÉS MARIANO; SBARAGLINI, MARÍA LAURA; RAMIREZ, EDUARDO ALEJANDRO; SCOLLO, KARENINA; VELA, MARÍA ELENA; SBARAGLINI, MARÍA LAURA; RAMIREZ, EDUARDO ALEJANDRO; SCOLLO, KARENINA; VELA, MARÍA ELENA

ACS Infectious Diseases

American Chemical Society

Lugar: Washington; Año: 2019 vol. 5 p. 1813 - 1819

2373-8227

Trans-sialidase and cruzipain are important virulence factors from Trypanosoma cruzi, the etiological agent of Chagas´ disease, that have highly antigenic domains in their structure and were reported as potential tools for diagnosis of the illness. The aim of the present study is to assess the possibility of using cruzipain and the catalytic domain of trans- sialidase in a Surface Plasmon Resonance-based immunosensor for the diagnosis of chronic Chagas´ disease. Immunoassays carried out with canine sera verified that cruzipain, allows the detection of anti- Trypanosoma cruzi antibodies whereas recombinant trans-sialidase did not yield specific detections, due to the high dilutions of serum used in the immunoassays that hinders the possibility to sense the specific low titer-antibodies. The developed cruzipain- based biosensor, whose price per assay is comparable to commercial ELISA, was successfully applied for the rapid quantification of specific antibodies against Trypanosoma cruzi in fresh human sera showing an excellent agreement with ELISA.