Fluorescent silica nanoparticles with chemically reactive surface:

M. LAURA VERA; FELIX G. REQUEJO; CRISTIÁN HUCK IRIART; ALEJANDRA CALVO; MARÍA L. DELL´ARCIPRETE ; ANTONELA CANNEVA; MÓNICA C. GONZALEZ

JOURNAL OF COLLOID AND INTERFACE SCIENCE

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2017 vol. 496 p. 456 - 494

0021-9797

The encapsulation of fluorescent dyesinside silica nanoparticles is advantageous to improve their quality as probes. Inside the particle, the fluorophore isprotected from the external conditions and its main emission parameters remainsunchanged even in the presence of quenchers. On the other hand, theamine-functionalized nanoparticle surface enables a wide range of applications,as amino groups could be easily linked with different biomolecules fortargeting purposes. This kind of nanoparticle is regularly synthesized by methodsthat employ templates, additional nanoparticle formation or multiple pathwayprocess. However, a one-step synthesis will be an efficient approach in thissort of bifunctional hybrid nanoparticles.A co-condensation sol-gel synthesisof hybrid fluorescent silica nanoparticle where developed. The chemical and morphologicalcharacterization of the particles where investigated by DRIFTS, XPS, SEM andSAXS. The nanoparticle fluorescent properties were also assessed byexcitation-emission matrices and time resolved experiments.We have developed a one-pot synthesis methodthat enables the incorporation of both functionalities by controllingco-condensation process. An exhaustive characterization allows the definitionof the spatial distribution of both functionalities: the fluorescent probe, fluoresceinisothiocyanate, inside the particle and reactive amino groups on the surface innanoparticle with diameter below 100 nm