Assessment of cellular effect in the vicinity of Mg, AZ31 and ZEK100 alloys

GRILLO, C; ALVAREZ, F; MULLER W-D; FERNÁNDEZ LORENZO DE MELE, M

EUROPEAN CELLS AND MATERIALS (ECM)

SWISS SOC BIOMATERIALS

Año: 2013 vol. 26S p. 42 - 42

1473-2262

The degradation process of Mg based resorbable implant materials induces biocompatibility problems detected at the implantation site. Cellular effects assessment showed that adverse effects may be associated to Mg and alloying ions when the limit of tolerance is surpassed1. It is worth noting that the concentration of released ions is not uniform in space and that toxic effect may be dependent on the distance from the source of ions. Metal salts and extracts obtained after the immersion of metal samples in culture media are frequently used to assess toxicity of the released metal ions in cell lines. However, the effect of the local environment close to the metal cannot be evaluated in this way. The aim of this work is to assess the change of the viability and the capacity of forming colonies of the cells in presence of Mg-based metal disks of different compositions with the distance from the source of ions. Results showed a strong cytotoxic effect in case of pure Mg, probably due to local changes related to the higher corrosion rate (pH, hydrogen burbles, high Mg ions concentration)1. In case of ZEK100 the decrease in the colony diameters suggests that its alloying elements may produce a more significant effect on the proliferation capacity of cells than AZ31.