CEPAVE 05420
CENTRO DE ESTUDIOS PARASITOLOGICOS Y DE VECTORES
Unidad Ejecutora - UE
artículos
Título:
Efecto de la calidad del agua de criaderos de mosquitos sobre la patogenicidad e infectividad de las zooporas del hongo Leptolegnia chapmanii (Straminipila: Peronosporomycetes)
Autor/es:
PELIZZA, S.A. LOPEZ LASTRA, C.C., MACIÀ, A., BISARO, V. Y GARCIA, J.J.
Revista:
REVISTA DE BIOLOGÃA TROPICAL
Editorial:
REVISTA DE BIOLOGIA TROPICAL
Referencias:
Lugar: San Jose de Costa Rica; Año: 2009 vol. 57 p. 371 - 380
ISSN:
0034-7744
Resumen:
Abstract: Effect of water quality in mosquito breeding sites on the pathogenicity and infectivity of zoospores
from the fungus Leptolegnia chapmanii (Straminipila: Peronosporomycetes). The fungus Leptolegnia
chapmanii is highly pathogenic to mosquito larvae in Argentina. We studied if physical and chemical characteristics
of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L.
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L.
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L.
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii is highly pathogenic to mosquito larvae in Argentina. We studied if physical and chemical characteristics
of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L.
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L.
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L.
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii is highly pathogenic to mosquito larvae in Argentina. We studied if physical and chemical characteristics
of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L.
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L.
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25 Aedes aegypti larvae, and 2.8 x 105 zoospores of
filled by overflow from Río de la Plata, and flower vases from the Cemetery of La Plata city. Sub-samples of
water were analyzed for physical and chemical characteristics, while other sub-samples were used for laboratory
bioassays. Containers with 150 ml of water samples, 25
