EUCALYPTUS PROTEOME RESPONSES TO COLD ACCLIMATION

OBERSCHELP, J.; HARRAND, L.; MARGARIT, E.; TESÓN, N.; GUARNASCHELLI, A.; PODESTÁ, F.E.

Cordoba

Congreso; XXXII Reunión Argentina / XVI Congreso Latinoamericano de FISIOLOGÍA VEGETAL; 2018

SAFV - Sociedad Argentina de Fisiología Vegetal

Cold acclimation is an adaptive process that leads toa transient increase in freezing tolerance. This processcomprises anatomical, physiological, and biochemicalchanges in plant cells in response to environmentalchanges. Previously, acclimation treatments were appliedto seedlings of three Eucalyptus species, and theireffects evaluated with frost assays. Eucalyptus benthamiishowed the highest tolerance, while Eucalyptusgrandis was the most sensitive. Biochemical characterizationindicated that E. benthamii had higher levelsof soluble sugars, phenolics and anthocyanins, whichwere possibly contributing to the observed tolerance.In this work, proteome characterization of these twospecies under control and cold acclimation treatmentswas performed in order to deepen our knowledge bothon the differences between species and on their acclimationresponses. Leaf proteins were extracted, submittedto untargeted proteome analysis, andbioinformatically characterized. As a result, 1163 proteinswhose levels significatively varied were identified,being interspecific comparison the one with the greatestnumber of proteins (ca. 700). Gene ontology andpathway characterization allowed the identification ofproteins related to primary and secondary metabolism,response to abiotic stimuli and stress. Proteomicshighlighted differences between species which mightexplain the observed cold tolerance and open new possibilitiesfor tree breeding.