Long-term effects of sucrose consumption on glycemia and rage expression in juvenile versus adult rats.

GONZÁLEZ DENISELLE, M. C.; KRUSE, M. S.; COIRINI, H.; REY, M.

Mar del Plata

Congreso; Reunión Anual de Biociencia 2019 - SAIC-SAFE-SAB-SAP-NANOMEDar-AACyTAL-HSC; 2019

Sociedad Argentina de Investigación Clínica

Excessive consumption of sucrose in earlystages of development has deleterious neurobiological andbehavioral effects in adulthood. Among these disturbances,difficulties in memory retrieval by early sucrose exposure werepreviously described. Here, we examined the glycemic levels ofthe animals and their response to an intraperitoneal glucoseoverload. We found that sucrose consumption increased the AUC(area under the curve derived from the oral glucose tolerancetest) in both young and adults (two-way ANOVA, FTreat(1,34)=7,658; p= 0.0091. Fisher's LSD post hoc test, p= 0.047 and p=0.040, respectively) but only consumption during youthmaintained this effect in the long term (two-way ANOVA,FTreat(1,34)= 4,445; p= 0.0429. Fisher's LSD post hoc test, p=0.020). RAGE expression was also assessed by Western blot. Agedifferences were detected by two-way ANOVA in the two brainareas examined, the mPFC and the vHIP, but only in the mPFCdifferences by treatment were observed (two-way ANOVA,FTreat-Age(1,20)= 2,327; p= 0.001). Surprisingly, sucroseexposed animals in their youth showed decrease of RAGE whileadults raised these values in the mPFC (Fisher's LSD post hoctest, p= 0.037 and p= 0.002, respectively). When all animalswere pulled together, there was a negative correlation of theexploration ratio of the memory recognition test and the RAGElevels in the mPFC (F(1,14)= 7,225; p= 0.0434; r2= 0.59)indicating that higher RAGE values relate with poorer noveltyrecognition on the final task of the memory test. Similarly, thebasal plasma glucose levels also correlated with lowerexploration ratio on the final recognition task (F(1,21)= 5,744;p= 0.0264; r2= 0.223). When basal glycaemia was analyzedtogether with mPFC RAGE levels, a positive correlation wasobserved (F(1,18)= 5,693; p= 0.0289; r2= 0.251). In summary,these results suggest that sucrose induced-hyperglycemia isdetrimental for the memory a phenomenon that is related withthe RAGE pathway in the mPFC.