Platelet-derived growth factor B (PDGFB) improves follicular development and ovarian angiogenesis in rar model of polycystic Ovary Syndrome

M. DI PIETRO, L. SCOTTI, G. IRUSTA, N. PASCUALI, M. TESONE, F.A PARBORELL, D. ABRAMOVICH.

Roma

Congreso; 17 th World Congress of the Academy of Human Reproduction; 2017

The International Academic Human Reproduction

PLATELET-derived growth factor B (PDGFB) improves follicular development and ovarian angiogenesis in rar model of polycystic Ovary SyndromeMariana Di Pietro, Leopoldina Scotti, Griselda Irusta, Natalia Pascuali, Marta Tesone, Fernanda Parborell, Dalhia Abramovich. Instituto de Biología y Medicina Experimental (IByME-CONICET). Context: Patients with Polycystic Ovary Syndrome (PCOS) have alterations in ovarian angiogenesis. The most studied of these alterations is the increase in vascular endothelial growth factor (VEGF) production by ovarian cells. Platelet-derived growth factor B and D (PDGFB and D) are decreased in follicular fluid of PCOS patients and in the ovaries of a rat model of PCOS. Therefore, we hypothesized that restoration of ovarian PDGFB levels can improve ovarian angiogenesis and follicular development in a rat model of PCOS. Objective: We aimed to analyze the effects of local administration of PDGFB on ovarian angiogenesis, follicular development and ovulation in a DHEA-induced PCOS rat model. Methods: Prepubertal female SD rats were injected sc with DHEA (6 mg per 100 g body weight) daily for 15 d to induce PCOS. Control animals were injected with 0.2 ml of sesame oil. For PDGFB administration, DHEA-treated rats were injected under the bursa of both ovaries with 25 ng of PDGFB on day 15 of DHEA treatment. Another group received saline. On day 16 rats were sacrificed and the ovaries collected for further studies. Main Outcome measures: follicular development, AMH, endothelial and periendothelial cell areas, angiogenic factor levels. Results: Ovarian PDGFB administration to PCOS rats partially restored follicular development, decreased the percentage of cysts and AMH levels and increased the percentage of corpora lutea. In addition, PDGFB improved ovarian angiogenesis by reversing the increase in periendothelial cell area and restoring VEGF levels. Conclusion: This study demonstrates a beneficial effect of local PDGFB administration on luteal development and ovarian angiogenesis in a rat model of PCOS. These results contribute to the knowledge of the ovarian mechanisms that lead to altered ovarian function in PCOS and provide new data for potential therapeutic strategies.