Actin cytoskeleton changes in neotriatum rats after hypoxia.

SARRACENO, E.; BOTI, V.; AON, L.; VALVERDE, G.DE ANDRADE, D.; MADUREIRA DE OLIVEIRA, D.; SAMPAIO, G.E.; GIRALDEZ ALVAREZ, L.D.; COIRINI, H.; CAPANI, F.

Instituto de Biología y Medicina Experimental, Vuelta de Obligado 2490, Ciudad Autónoma de Buenos Aires, Argentina

Congreso; VIII Jornadas Multidisciplinarias de la Sociedad Argentina de Biología; 2006

Sociedad Argentina de Biología

Dendritic spines are highly concentrated in F-actin being this protein involved in the movements and shape changes of these structures after several types of physiological conditions. Several proteins involved in synaptic functions are damaged by hypoxia-insult. These alterations might destroy synaptic transmission and then induce neurological deficits. In previous report we have  shown alterations in synaptic organization after hypoxia. In the present studied we have investigated the effect hypoxia on actin cytoskeleton distribution in neostriatum from 60 days old rats subjected to 20 minutes of severe hypoxia at 37 °C during the birth. We have used correlative phalloidin photoconvertion and 3-D serial sections reconstructions to conduct this study. Under severe PA conditions confocal microscopy for phalloidin-Alexa567 showed an  increment of F-actin fluorescent staining. In addition, electron microscopy and 3-D reconstruction confirmed these observations showing an increment of F-actin staining in neostriatal excitatory synapses subjected to hypoxia. Therefore since F-actin is highly concentrated in excitatory synapses we think that actin changes could be related with the increment in the release of excitatory aminoacids, and then in the neuronal death. increment of F-actin fluorescent staining. In addition, electron microscopy and 3-D reconstruction confirmed these observations showing an increment of F-actin staining in neostriatal excitatory synapses subjected to hypoxia. Therefore since F-actin is highly concentrated in excitatory synapses we think that actin changes could be related with the increment in the release of excitatory aminoacids, and then in the neuronal death. showed an increment of F-actin fluorescent staining. In addition, electron microscopy and 3-D reconstruction confirmed these observations showing an increment of F-actin staining in neostriatal excitatory synapses subjected to hypoxia. Therefore since F-actin is highly concentrated in excitatory synapses we think that actin changes could be related with the increment in the release of excitatory aminoacids, and then in the neuronal death. showed an increment of F-actin fluorescent staining. In addition, electron microscopy and 3-D reconstruction confirmed these observations showing an increment of F-actin staining in neostriatal excitatory synapses subjected to hypoxia. Therefore since F-actin is highly concentrated in excitatory synapses we think that actin changes could be related with the increment in the release of excitatory aminoacids, and then in the neuronal death. showed an increment of F-actin fluorescent staining. In addition, electron microscopy and 3-D reconstruction confirmed these observations showing an increment of F-actin staining in neostriatal excitatory synapses subjected to hypoxia. Therefore since F-actin is highly concentrated in excitatory synapses we think that actin changes could be related with the increment in the release of excitatory aminoacids, and then in the neuronal death.