IFEVA   02662
INSTITUTO DE INVESTIGACIONES FISIOLOGICAS Y ECOLOGICAS VINCULADAS A LA AGRICULTURA
Unidad Ejecutora - UE
artículos
Título:
Thermodormancy and ABA metabolism in barley grains
Autor/es:
JULIETTE LEYMARIE, ROBERTO L. BENECH-ARNOLD, JILL M. FARRANT AND FRANÇOISE CORBINEAU
Revista:
Plant Signaling & Behavior
Editorial:
Landes Bioscience Journals
Referencias:
Año: 2009 vol. 4 p. 205 - 207
ISSN:
1559-2316
Resumen:
Incubation of barley primary dormant grains at 30°C, a temperature
at which they cannot germinate results in a reinforcement of
their sensitivity to temperature, and in particular in a loss of their
ability to germinate at 1520°C.1 Incubation of the grains at 30°C
in the presence of GA3 (1 mM) or of isolated embryos prevents
this induction of secondary dormancy. In such a condition, embryo
ABA content was lower than that measured in embryos of seeds
incubated at 30°C on water. Expression of genes involved in ABA
metabolism (HvABA8'OH1, HvNCED1 and HvNCED2) was
studied in isolated embryos incubated on water and in the presence
of GA3 (1 mM). Expression of HvABA8'OH1, HvNCED11 Incubation of the grains at 30°C
in the presence of GA3 (1 mM) or of isolated embryos prevents
this induction of secondary dormancy. In such a condition, embryo
ABA content was lower than that measured in embryos of seeds
incubated at 30°C on water. Expression of genes involved in ABA
metabolism (HvABA8'OH1, HvNCED1 and HvNCED2) was
studied in isolated embryos incubated on water and in the presence
of GA3 (1 mM). Expression of HvABA8'OH1, HvNCED13 (1 mM) or of isolated embryos prevents
this induction of secondary dormancy. In such a condition, embryo
ABA content was lower than that measured in embryos of seeds
incubated at 30°C on water. Expression of genes involved in ABA
metabolism (HvABA8'OH1, HvNCED1 and HvNCED2) was
studied in isolated embryos incubated on water and in the presence
of GA3 (1 mM). Expression of HvABA8'OH1, HvNCED1HvABA8'OH1, HvNCED1 and HvNCED2) was
studied in isolated embryos incubated on water and in the presence
of GA3 (1 mM). Expression of HvABA8'OH1, HvNCED13 (1 mM). Expression of HvABA8'OH1, HvNCED1
and HvNCED2 was discussed in relation with ABA content and
germination ability at 30°C.HvNCED2 was discussed in relation with ABA content and
germination ability at 30°C.