STNDPK3 IS A MITOCHONDRIAL NUCLEOSIDE DIPHOSPHATE KINASE FROM Solanum tuberosum

GROSSI CECILIA EUGENIA MARÍA; FANTINO ELISA; ULLOA RITA MARÍA; SANTIN FRANCO

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAA-SAB-SAIC-SAIB-SAI-SAFE-SAH-SAP-SAB-SAFIS

Nucleoside diphosphate kinases (NDPKs) are capable of altering the set of nucleoside triphosphates, participating in the intracellular energy communication network and have been described in several signaling cascades associated to stress responses in plants. Plant NDPKs are organized in four distinct types (I to IV) according to their subcellular localization and their phylogenetic relationships. The cytosolic isoform Type I NDPK (NDPK1) has been shown to be the major NDPK isoform in plants (Dorion et al., 2006). An in silico search of theSolanum phureja genome (PGSC) and ClustalW alignment with NDPKs from other plants, animals and microorganisms allowed us to identify two chloroplastic (type II) and two mitochondrial (type III) isoforms that belong to group I and one secreted isoform (type IV) that belongs to group II. In this work we characterize one of the potato mitochondrial isoforms. The complete coding sequence of StNDPK3 was cloned from potato sprouts and the recombinant protein was expressed. The analysis of its expression profile reveals that it is an ubiquitous isoform. RT-qPCR assays of plants infected with Phytopthora infestans suggest that it could be involved in biotic responses.