Characterization of Protein Phosphatases Type 2A in Solanaceae”

PAÍS S.M,; GONZALEZ M.A.,; TÉLLEZ-IÑÓN M.T.; CAPIATI D

Rosario, Argentina

Congreso; XLII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology (SAIB).; 2006

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).

Cha Protein phosphorylation/dephosphorylation plays critical roles in growth and development of plants, being serine/threonine phosphorylation the predominant postranslational modification. Ser/Thr phosphatases include phosphatases type 1, 2A and 2C. PP2A contain a highly conserved catalytic subunit, a structural subunit (A) and a regulatory subunit (B). A potato phosphatase type 2A (StPP2A1c) was described previously in our lab and evidence on the participation of this isoform in tuber development was obtained. In tomato plants, changes in PP2A levels under abiotic stress were observed suggesting the participation of one or more isoforms in the stress response. The aim of this study was to characterize the catalytic subunit of PPase 2A present in potato (Solanum tuberosum) and tomato (Solanum lycopersicum). Sequence homology analysis revealed six isoforms in potato and five in tomato. Presence of functional domains, prediction of subcellular localization and phylogenetic analysis were carried out using bioinformatics analysis. Expression pattern profiles of PP2A isoforms in potato and tomato under different environmental conditions (mainly stimuli related to tuber development for potato and abiotic stress for tomato) were determined. Bioinformatics and expression profile analysis yielded evidence on the physiological roles of different isofoms of PP2A in potato and tomato. racterization of Protein Phosphatases Type 2A in Solanaceae”