Evaluation of the interaction between different liposome preparation and Trypanosoma cruzi developmental stages.

DIBLASI LORENA; HERMIDA ; PORTAL PATRICIO; PAVETO CRISTINA; ROSI PABLO

Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas y Biología Molecular (SAIB); 2010

Chagas’ disease, one of the major health problems in Latin America, is caused by the kinetoplastid protozoan Trypanosoma cruzi. Transmission occurs mainly by triatominae insect vectors, but also by blood transfusion and congenital routes. Although successful programs in vector control and banked-blood screening have led to a decline in transmission, neither total eradication nor effective treatment of the disease has been achieved. The only current treatment relies on the use of nifurtimox and benznidazole, both drugs with toxic side effects and limited efficacy in chronic patients. The main challenges in the pharmacotherapy of Chagas’ disease are to increase effectiveness of drug treatment and to prevent adverse effects. Nano-drug delivery systems (nanoDDS) represent useful means to selectively deliver the drug to intracellular targets. In this work we assayed the interaction of different lipid composition and sizes of liposomes with epimastigotes, blood stream tripomastigote and amastigote form of T.cruzi. As phosphatidylcholine (PC) is the major and widely used component of lipidic nanoparticles, we prepared egg PC nnDDS vs soy bean PC and could observed that the source of PC preparation modifies the interaction of liposome preparation with the parasitic cell. The response of this interaction depends on the nnDDS composition and particle size, and varies on the parasite cell stage.