Trypanosoma cruzi I genotypes in different geographic regions and transmission cycles

CAROLINA I. CURA; ANA M. MEJÍA-JARAMILLO; TOMÁS DUFFY; JUAN M. BURGOS; MARCELA RODRIGUERO; MARTA V. CARDINAL; SONIA KJOS; RODRIGO GURGEL-GONÇALVES; DENIS BLANCHET; LUIS M. DE PABLOS; NICOLÁS TOMASINI; ALEX DA SILVA; GRACIELA RUSSOMANDO; CESAR A. CUBA CUBA; CHRISTINE AZNAR; TERESA ABATE; MARIANO JORGE LEVIN; ANTONIO OSUNA; RICARDO E. GÜRTLER; PATRICIO DIOSQUE; ALDO SOLARI; OMAR TRIANA CHAVEZ; ALEJANDO GABRIEL SCHIJMAN

Atlanta

Congreso; American Society of Tropical Medicine and Hygiene 59th Annual Meeting.; 2010

American Society of Tropical Medicine and Hygiene

The intergenic region of spliced-leader (SL-IR) genes from 105 Trypanosoma cruzi Iinfected biological samples, culture isolates and stocks from 11 endemic countries, fromArgentina to USA, were characterised, allowing identification of 76 genotypes with 54polymorphic sites from 123 aligned sequences. On the basis of the microsatellite motifproposed by Herrera et al. (2007) to define four haplotypes in Colombia, we couldclassify these genotypes into four distinct Tc I SL-IR groups, three corresponding to theformer haplotypes Ia (11 genotypes), Ib (11 genotypes) and Id (35 genotypes); and onenovel group, Ie (19 genotypes). Tc Ia was associated with domestic cycles in Southernand Northern South America and sylvatic cycles in Central and North America. Tc Ibwas found in all transmission cycles from Colombia. Tc Id was identified in alltransmission cycles from Argentina and Colombia, including Chagas cardiomyopathypatients, sylvatic Brazilian samples and human cases from French Guiana, Panama andVenezuela. Tc Ie gathered five samples from domestic Triatoma infestans from NorthernArgentina, nine samples from wild Mepraia spinolai and Mepraia gajardoi and twochagasic patients from Chile and one from a Bolivian patient with chagasic reactivation.Mixed infections by Tc Ia + Tc Id, Tc Ia + Tc Ie and Tc Id + Tc Ie were detected invector faeces and isolates from human and vector samples. In addition, Tc Ia and Tc Idwere identified at different tissues from a heart transplanted Chagas cardiomyopathypatient with reactivation, denoting histotropism.T. cruzi I SL-IR genotypes from parasites infecting Triatoma gerstaeckeri and Didelphisvirginiana from USA, T. infestans from Paraguay, Rhodnius nasutus and Rhodniusneglectus from Brazil and M. spinolai and M. gajardoi from Chile are described for thefirst time