INGEBI 02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
Organization of Circadian Behavior Relies on Glycinergic Transmission
Autor/es:
NARA I MURARO; GUILLERMO BERNABÓ; CHARLOTTE HELFRICH-FÖRSTER; DANIEL J. CALVO; LIA FRENKEL; MARIA SILVINA MÁRCORA; JUAN I. ROMERO; CRISTINA MARINO-BUSJLE; ANDREA N BELTRÁN GONZALEZ; CHRISTIANE HERMANN-LUIBL; EDUARDO M. CASTAÑO; M. FERNANDA CERIANI
Revista:
Cell Reports
Editorial:
Elsevier
Referencias:
Lugar: Ámsterdam; Año: 2017 vol. 19 p. 72 - 85
ISSN:
2211-1247
Resumen:
The small ventral lateral neurons (sLNvs) constitute acentral circadian pacemaker in the Drosophila brain.They organize daily locomotor activity, partly throughthe release of the neuropeptide pigment-dispersingfactor (PDF), coordinating the action of the remainingclusters required for network synchronization.Despite extensive efforts, the basic principlesunderlying communication among circadian clustersremain obscure. We identified classical neurotransmittersreleased by sLNvs through disruption ofspecific transporters. Adult-specific RNAi-mediateddownregulation of the glycine transporter or impairmentof glycine synthesis in LNv neurons increasedperiod length by nearly an hour without affectingrhythmicity of locomotor activity. Electrophysiologicalrecordings showed that glycine reduces spikingfrequency in circadian neurons. Interestingly, downregulationof glycine receptor subunits in specificsLNv targets impaired rhythmicity, revealing involvementof glycine in information processing within thenetwork. These data identify glycinergic inhibitionof specific targets as a cue that contributes to thesynchronization of the circadian network.
