INGEBI 02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
2-C-Methyluridine modified hammerhead ribozyme against the Estrogen Receptor alpha
Autor/es:
RODRIGO PONTIGGIA, OSVALDO PONTIGGIA, MARINA SIMIAN, JAVIER M. MONTSERRAT, JOACHIM W. ENGELS, ADOLFO M. IRIBARREN
Revista:
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2010 vol. 20 p. 2806 - 2808
ISSN:
0960-894X
Resumen:
A new synthesis of 2-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the Estrogen Receptor a protein (ER-a), and transfection experiments in MCF-7 cell line are also presented
