INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
artículos
Título:
A CDPK isoform participates in the regulation of nodule number in Medicago truncatula
Autor/es:
PABLO GARGANTINI; SILVINA GONZALEZ-RIZZO; DELPHINE CHINCHILLA; MARCELA RAICES; VERÓNICA GIAMMARIA; RITA M. ULLOA; FLORIAN FRUGIER; MARTIN CRESPI
Revista:
PLANT JOURNAL
Editorial:
Blackwell University Press
Referencias:
Lugar: Oxford; Año: 2006 vol. 48 p. 843 - 856
ISSN:
0960-7412
Resumen:
Summary
Medicago spp. are able to develop root nodules via symbiotic interaction with Sinorhizobium meliloti.
Calcium-dependent protein kinases (CDPKs) are involved in various signalling pathways in plants, and we
found that expression of MtCPK3, a CDPK isoform present in roots of the model legume Medicago truncatula,
is regulated during the nodulation process. Early inductions were detected 15 min and 34 days postinoculation
(dpi). The very early induction of CPK3 messengers was also present in inoculated M. truncatula
dmi mutants and in wild-type roots subjected to salt stress, indicating that this rapid response is probably
stress-related. In contrast, the later response was concomitant with cortical cell division and the formation of
nodule primordia, and was not observed in wild-type roots inoculated with nod ) strains. This late induction
correlated with a change in the subcellular distribution of CDPK activities. Accordingly, an anti-MtCPK3
antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are
targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and
palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were
also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other
nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium
rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.spp. are able to develop root nodules via symbiotic interaction with Sinorhizobium meliloti.
Calcium-dependent protein kinases (CDPKs) are involved in various signalling pathways in plants, and we
found that expression of MtCPK3, a CDPK isoform present in roots of the model legume Medicago truncatula,
is regulated during the nodulation process. Early inductions were detected 15 min and 34 days postinoculation
(dpi). The very early induction of CPK3 messengers was also present in inoculated M. truncatula
dmi mutants and in wild-type roots subjected to salt stress, indicating that this rapid response is probably
stress-related. In contrast, the later response was concomitant with cortical cell division and the formation of
nodule primordia, and was not observed in wild-type roots inoculated with nod ) strains. This late induction
correlated with a change in the subcellular distribution of CDPK activities. Accordingly, an anti-MtCPK3
antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are
targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and
palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were
also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other
nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium
rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.MtCPK3, a CDPK isoform present in roots of the model legume Medicago truncatula,
is regulated during the nodulation process. Early inductions were detected 15 min and 34 days postinoculation
(dpi). The very early induction of CPK3 messengers was also present in inoculated M. truncatula
dmi mutants and in wild-type roots subjected to salt stress, indicating that this rapid response is probably
stress-related. In contrast, the later response was concomitant with cortical cell division and the formation of
nodule primordia, and was not observed in wild-type roots inoculated with nod ) strains. This late induction
correlated with a change in the subcellular distribution of CDPK activities. Accordingly, an anti-MtCPK3
antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are
targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and
palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were
also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other
nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium
rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.CPK3 messengers was also present in inoculated M. truncatula
dmi mutants and in wild-type roots subjected to salt stress, indicating that this rapid response is probably
stress-related. In contrast, the later response was concomitant with cortical cell division and the formation of
nodule primordia, and was not observed in wild-type roots inoculated with nod ) strains. This late induction
correlated with a change in the subcellular distribution of CDPK activities. Accordingly, an anti-MtCPK3
antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are
targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and
palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were
also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other
nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium
rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.mutants and in wild-type roots subjected to salt stress, indicating that this rapid response is probably
stress-related. In contrast, the later response was concomitant with cortical cell division and the formation of
nodule primordia, and was not observed in wild-type roots inoculated with nod ) strains. This late induction
correlated with a change in the subcellular distribution of CDPK activities. Accordingly, an anti-MtCPK3
antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are
targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and
palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were
also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other
nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium
rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.nod ) strains. This late induction
correlated with a change in the subcellular distribution of CDPK activities. Accordingly, an anti-MtCPK3
antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are
targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and
palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were
also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other
nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium
rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.MtCPK3
antibody detected two bands in soluble root extracts and one in the particulate fraction. CPK3::GFP fusions are
targeted to the plasma membrane in epidermal onion cells, a localization that depends on myristoylation and
palmitoylation sites of the protein, suggesting a dual subcellular localization. MtCPK3 mRNA and protein were
also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other
nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium
rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.MtCPK3 mRNA and protein were
also up-regulated by cytokinin treatment, a hormone linked to the regulation of cortical cell division and other
nodulation-related responses. An RNAi-CDPK construction was used to silence CPK3 in Agrobacterium
rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.Agrobacterium
rhizogenes-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.-transformed roots. Although no major phenotype was detected in these roots, when infected with
rhizobia, the total number of nodules was, on average, twofold higher than in controls. This correlates with the
lack of MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.MtCPK3 induction in the inoculated super-nodulator sunn mutant. Our results suggest that CPK3
participates in the regulation of the symbiotic interaction.
Keywords: CDPK, Medicago truncatula, nodulation, cytokinins, stress.Medicago truncatula, nodulation, cytokinins, stress.