FullSSR: a new approach for primer design from next-generations sequencing data

METZ, SEBASTIÁN; CABRERA, JUAN MANUEL; GIRI, FEDERICO; RUEDA, EVA; AMAVET, PATRICIA

Rosario

Congreso; 4to. Congreso Argentino de Bioinformática y Biología Computacional (4CAB2C) y 4ta. Conferencia Internacional de la Sociedad Iberoamericana de Bioinformática (SolBio); 2013

CIFASIS-CONICET- UNR- AMU

Traditional methods for isolation of microsatellites (SSRs) are often expensive and time consuming. Nowadays is easier and cheaper to obtain genomic data from next generation sequencing. Then, at least two softwares need to be applied in order to obtain SSR primers of SSR flanking sequences for PCR assays. Here we propose new software wich uses a simple language and allows the user to work with several contigs for PCR primer designe in one step.